University Animal Care Committee Standard Operating Procedure
Document No: 7.5.1
Subject: Humane Killing Method for Rodents via Barbiturate or Injectable Anaesthetic
Date Issued: August 21, 2012
Revision: 3
Location: Queen’s University
Responsibility: Principal Investigators (PI), Research Staff, Veterinary Staff
Purpose: The purpose of this Standard Operating Procedure (SOP) is to describe the use of barbiturates or injectable anesthesia for humanely killing an animal.
1. Introduction and Definitions:
As per the UACC policy on humane killing of animals used in science, overdose of barbiturates or injectable anaesthetic is an acceptable method of humane killing for rodents when conducted properly. Unless justified and approved in the Animal Use Protocol, a Primary method (rendering death) of humane killing must be followed by a Secondary (confirmation of death) method.
Abbreviations: Animal Care Services ACS, Principal Investigator PI, subcutaneous SC, intravenous IV, intraperitoneal IP, intramuscular IM, per os PO, per rectum PR
2. Materials:
- Sterile needles (assorted sizes – 23-27g)
- Sterile syringes (assorted sizes 1-10ml)
- Barbiturate or injectable anaesthesia
3. Procedures:
- Using an appropriate gauge needle, restrain the animal gently and inject one of the following intraperitoneally:
- Three times the calculated anesthetic dose or;
- 100mg/kg sodium pentobarbital.
- Following the injection, the animal should be placed back in its cage (lined with paper to avoid inhalation of bedding material), and placed in a quiet area to minimize excitement and trauma until humane killing is complete. Multiple animals should not be placed within the same cage during anesthesia.
- To confirm death, monitor the animal for the following signs: cessation of chest movement, no palpable heartbeat, poor mucous membrane color, no response to toe pinch, and color change/opacity of the eyes.
- A secondary method of humane killing can be completed at this time, such as
bilateral pneumothorax, cervical dislocation, or exsanguination. - Carcasses should be disposed of promptly.
**A physical method of humane killing such as a bilateral pneumothorax, cervical dislocation, or exsanguination is required before disposal of the animal to confirm humane killing. The handler must ensure complete separation between the cervical vertebrae and skull (severance of the spinal cord) if cervical dislocation is the secondary method.**
, Volume 45, Number 1, January 2006, pp. 98-105(8) James Artwohl, Patricia Brown, Brian Corning and Susan Stein Report of the ACLAM Task Force on Rodent Euthanasia
| Date | New Version |
|---|---|
| 02/28/2019 | Update |
| 02/28/2022 | Triennial Update |
| 07/22/2024 | Triennial Update - updated format, removed neonate euthanasia, changed wording (humane killing) |